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KMID : 0981820090290040269
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Korean Journal of Laboratory Medicine
2009 Volume.29 No. 4 p.269 ~ p.276
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Detection of Putative T cell Clones Using T cell Receptor βChain Gene Clonality Assay in Korean Patients with Aplastic Anemia
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Choi Hyun-Jung
Kook Hoon
Kim Hyeoung-Joon
Shin Myung-Geun
Kee Seung-Jung
Shin Jong-Hee
Suh Soon-Pal
Ryang Dong-Wook
Kim Soo-Hyun
Kim Hye-Ran
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Abstract
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Background: We analyzed T cell receptor β chain (TCRB) gene to investigate the presence of putative T cell clones and its clinicopathologic implications in Korean patients with aplastic anemia (AA).
Methods: Twenty-nine bone marrow specimens were collected from 20 AA patients, 19 specimens from initial diagnosis and 10 from follow-up. T cell clonality assay was performed using IdentiClone™ TCRB Gene Clonality Assay kit (InVivoScirbe Technology, USA) and automatic genetic analyzer. Patients’ clinical information and laboratory parameters were also analyzed.
Results: Five patients had definitive underlying factors related with aplastic anemia, such as hepatitis B virus (4 cases) and benzene exposure (1 case). Putative T cell clones were detected in bone marrow specimens of 11 (58%) out of 19 patients at diagnosis. The location of putative T cell clones of TCRB gene (diversity region, Dβ; joining region, Jβ; variable region, Vβ) was distributed in Dβ2+Jβ2 (6 cases), Dβ1+Jβ1 (3 cases), Vβ+Jβ1 (2 cases), and Dβ1+Jβ2 (2 cases). Interestingly, among seven patients who underwent stem cell transplantation, five patients with no T cell clones detected at diagnosis developed new T cell clones during the follow-up.
Conclusions: Putative pathogenetic T cell clones were detected in most of AA patients in the current study. T cell clonality assay would be useful for investigating the pathophysiology of acquired AA.
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KEYWORD
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Aplastic anemia, T cell receptor βchain gene, T cell clone
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